Field Laboratory Evaluation of the GeneXpert Ebola Assay for Diagnosis of Ebola Virus Disease in Sierra Leone
نویسندگان
چکیده
LB-1. Field Laboratory Evaluation of the GeneXpert Ebola Assay for Diagnosis of Ebola Virus Disease in Sierra Leone Amanda Semper, DPhil; M. Jana Broadhurst, MD, PhD; Jade Richards, BTEC; Geraldine M. Foster, PhD; Andrew Simpson, FRCPath; Elisabetta Groppelli, PhD; Julie Johnson, PhD; J. Dan Kelly, MD; Tim Brooks, FRCPath, FRSPH; Christopher H. Logue, PhD; Ann Miller, PhD; Megan Murray, MD, ScD; Nira R. Pollock MD, PhD; Phe Microbiology Services, Rare and Imported Pathogens Laboratory, Porton Down, United Kingdom; Partners In Health, Boston, Massachusetts; Public Health England Laboratory, Port Loko, Sierra Leone; Wellbody Alliance, Kono, Sierra Leone; Public Health England, Porton Down, United Kingdom; Harvard Medical School, Boston, Massachusetts; Boston Children’s Hospital, Boston, Massachusetts
منابع مشابه
Performance of the GeneXpert Ebola Assay for Diagnosis of Ebola Virus Disease in Sierra Leone: A Field Evaluation Study.
BACKGROUND Throughout the Ebola virus disease (EVD) epidemic in West Africa, field laboratory testing for EVD has relied on complex, multi-step real-time reverse transcription PCR (RT-PCR) assays; an accurate sample-to-answer RT-PCR test would reduce time to results and potentially increase access to testing. We evaluated the performance of the Cepheid GeneXpert Ebola assay on clinical venipunc...
متن کاملCOVID-19 Outbreak in Africa: Lessons and Insights from the West African Ebola Virus Disease Epidemics
Over the years, the African continent has had to battle several outbreaks of infectious diseases in different countries. Some of the most deadly were the Ebola virus disease (EVD) outbreaks that occurred in West Africa between 2014 and 2016 affecting Guinea, Liberia, and Sierra Leone and, more recently, from 2018 to 2020 in the Democratic Republic of Congo (DRC). In the era of the COVID-19 pand...
متن کاملSouth African Ebola diagnostic response in Sierra Leone: A modular high biosafety field laboratory
BACKGROUND In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. METHODS AND FINDINGS The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" ...
متن کاملNotes from The Field: Ebola Virus Disease Cluster - Northern Sierra Leone, January 2016.
On January 14, 2016, the Sierra Leone Ministry of Health and Sanitation was notified that a buccal swab collected on January 12 from a deceased female aged 22 years (patient A) in Tonkolili District had tested positive for Ebola virus by reverse transcription-polymerase chain reaction (RT-PCR). The most recent case of Ebola virus disease (Ebola) in Sierra Leone had been reported 4 months earlie...
متن کاملEvaluation of a point-of-care blood test for identification of Ebola virus disease at Ebola holding units, Western Area, Sierra Leone, January to February 2015.
Current Ebola virus disease (EVD) diagnosis relies on reverse transcription-PCR (RT-PCR) technology, requiring skilled laboratory personnel and technical infrastructure. Lack of laboratory diagnostic capacity has led to diagnostic delays in the current West African EVD outbreak of 2014 and 2015, compromising outbreak control. We evaluated the diagnostic accuracy of the EVD bedside rapid diagnos...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 2 شماره
صفحات -
تاریخ انتشار 2015